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Effect of fuzheng guben granule on haematogenesis function in tumor bearing mice after chemotherapy

Nan Chen, Linwei Chen, Su Jiang, Zichuan Wang, Tao Liu

Objective: To observe the effect of Fuzheng Guben Granule on haematogenesis function in tumor bearing mice after chemotherapy.

Methods: Lewis lung carcinoma (LLC) model was established in 48 cases of c57bl/6 mice adapting to alternate light and dark, and were divided into such six groups as blank group, model group, chemotherapy group and Traditional Chinese Medicine (TCM) at Chen Shi (7-9 a.m) plus chemotherapy group (referred to as Chenshi group), TCM at Wushi (11 a.m. to 1p.m.) plus chemotherapy group (referred to as Wushi group) and TCM at Haishi (9 p.m. to 11 p.m.) plus chemotherapy group (referred to as Haishi group) by random number table, with 8 cases in each group. Five days after successful model establishment, the subjects were treated with Fuzheng Guben Granule and cisplatin chemotherapy different times. After 2 weeks, they were killed at the same time with the samples like blood collected for detection of related indexes.

Results: Compared with model group, white blood cells and red blood cells decreased significantly in chemotherapy group, Chenshi group and Wushi group, and the difference was statistically significant (P<0.05). Compared with chemotherapy group, white blood cell count has rebounded in Chenshi, Wushi and Haushi groups with the difference being statistically significant (P<0.05). Compared with Wushi group, white blood cell count rebounded more significantly in Haishi group of statistical significance (P<0.05), but with no significant difference in between in red blood cell count (P>0.05). Compared with model group, karyocyte count in marrow decreased significantly of statistical value(P<0.05) in chemotherapy group, Chenshi group, Wushi group and Haishi group, in which the count decreased most significantly in chemotherapy group and has a varying degree of rebound in other three groups by contrast (P<0.05). Compared with Chenshi group and Wushi group, the karyocyte count in marrow rebounded significantly in Haishi group of statistical value (P<0.05). The levels of serum EPO and G-CSF decreased to varying degrees in all experimental groups, most significantly in chemotherapy group when compared with model group, and the difference was statistically significant (P<0.05). Compared with chemotherapy group, the levels of serum EPO and G-CSF increased significantly in Haishi group of statistical value (P<0.05). There were different degrees of serum EPO and G-CSF rebound in Chenshi, Wushi and Haishi groups, in which the rebound of G-CSF was more obvious in Haishi group than in Chenshi group of statistical significance, (P<0.05), but with no significant change in serum EPO level (P>0.05).

Conclusion: Fuzheng Guben Granule can improve chemotherapy induced bone marrow suppression in mice bearing Lewis lung cancer, and it produces varying effects on recovery of bone marrow suppression when different time is selected in its application, with the efficacy being most obvious at Haishi.