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Isoginkgetin inhibits lipopolysaccharide induced PI3K/AKT pathway through miRNA210 in vascular endothelial cells
Bin Yuan, Haomiao Rui, Huichao Zhang, Xuemin Wang, Hong Wu & Lihua HanObjective: To investigate whether isoginkgetin can inhibit lipopolysaccharide induced PI3K/ AKT pathway through miRNA210 in vascular endothelial cells.
Methods: The experiment was used isoginkgetin 20 uM to treat lipopolysaccharide induced endothelial cells and to observe the protein and gene expression of miRNA210 and phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathways. In addition, the effect of isoginkgetin on the PI3K/AKT signaling pathway was observed after the miRNA210 gene was overexpressed by the virus. The experiment mainly tested by PCR and Western blot to detect the gene and protein expression.
Results: The results showed that isoginkgetin could significantly inhibit the expression of miRNA210, PI3K and Akt in the vascular endothelial cells induced by lipopolysaccharide. The effect of isoginkgetin was significantly inhibited after the use of viral overexpression of miRNA210.
Conclusion: Isoginkgetin mainly inhibits the PI3K/AKT signaling pathway in lipopolysaccharide induced vascular endothelial cells by inhibiting miRNA210. In addition, miRNA210 may play an important role in many PI3K pathway related diseases.