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The effect of ulinastatin combined with bone marrow mesenchymal stem cells on directional repairing of liver ischemia and reperfusion injury in rats

Weixing Wang, Zhixiong Wu, Xidong Wang & Lianjie Liu

Objective: To investigate repairing effect of ulinastatin combined with bone marrow mesenchymal stem cells (MSCs) on liver ischemia and reperfusion injury in rats.

Methods: A total of 54 healthy rats were randomly divided into sham operation group, model group and treatment group with 18 rats included in each group. The Pringle ‘s method was adopted to establish ischemia-reperfusion rat model and in each group a quantity of 3 mL blood was extracted from abdominal aorta respectively at 1, 2, 3 and 24 hours after reperfusion (6 rats were taken at each point) followed by measurement of level changes of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), nitric oxide (NO) and endothelin-1 (ET-1); The specimen of ischemic lobes of liver was taken to detect the protein expression of Toll like receptor 4 (TLR4).

Results: The expression level of ALT, AST and MDA at 1, 2, 3 and 24-hour after reperfusion was significantly increased in model group and treatment group than those in the sham-operation group (P<0.05). Compared with the sham-operation group, the NO level in other two groups (it was higher in the treatment group than in the model group) was significantly lower (P<0.05) and the ET-1 level in other two groups (it was higher in the model group than in the treatment group) turned out to be notably higher (P<0.05); At 24 hours after reperfusion, TLR4 protein was detected in all three groups and its level was higher in the model group and the treatment group(it was higher in the model group than in the treatment group) than those in the sham operation group.

Conclusion: Pretreatment of ulinastatin combined with bone marrow mesenchymal stem cells changes serum ALT, AST, MDA, NO and ET-1 level after ischemia reperfusion injury, inhibits the expression of liver cell TLR4 and then has a protective effect on the ischemia reperfusion injury.